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Lactoferrine en Pharming

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[verwijderd] 8 februari 2017 06:47

Wat doet pharming hier nog mee?

Transgenic cows' milk containing rhLF at 1.2 g /L was provided by Pharming Group NV's herd in Wisconsin. Milk was collected from.....

www.google.nl/url?sa=t&source=web...

[verwijderd] 8 februari 2017 08:33

Al heel lang niets meer dus.

ps dit is een vraag die ook gewoon in een bestaand draadje gesteld kan worden. Voor de overzichtelijkheid zijn zo min mogelijk draadjes erg prettig.

[verwijderd] 8 februari 2017 08:52

Overigens is mijn opmerking van hierboven wat prematuur gemaakt blijkt nu na lezing van het artikel, waarvoor excuus.
Het blijkt een recente studie over de de glycosylatie-eigenschappen van recombinant lactoferrine, ten behoeve waarvan Pharming wat melk van haar transgene koeien ter beschikking heeft gesteld. Pharming mag er dan wel niet actief meer mee bezig zijn, diverse onderzoekers blijkbaar nog wel.

[verwijderd] 8 februari 2017 11:42

Conclusion

Interest in LF is related to its wide range of biological properties that make it a major component for infant development. However, the mechanisms involved have not been well elucidated, especially the role that is played by glycan patterns on biological functions of LF. The results of our study could enable future investigations of the effects of glycosylation on LF properties.

The methodology employed in this study allowed isolation of a protein from a complex mixture, followed by the identification of N-glycans with high resolution and an isomer-level separation. N-glycan compositions released from commercial enzyme PNGase F were identified and compared among three LFs (bLF, hLF and rhLF). The results revealed a high diversity of N-glycan structures, including fucosylated and sialylated complex glycans, that may have bioactive potential. This study also increased the knowledge on rhLF and showed that it is a good substitute of bLF in bovine-based food. Transgenic cow’s milk that contain 1.2 g/L of rhLF could also be a good substrate for another novel enzyme called Endo-ß-N-acetylglucosaminidase (EndoBI-1) of proteins [42–45]. Releasing higher amounts of lactoferrin’s glycans together with the un-glycosylated protein bone in its native (non-denatured) state will help understand the real contributions of N-glycans to lactoferrin’s functional activities.

LF supplementation has already many applications in food and non-food products around the world and its market continues to grow. The synthesis of rhLF via transgenic cows appears as a suitable production method that provides a large amount of rhLF with high homology with hLF. Numerous studies will be necessary to highlight the efficacy and safety of rhLF as a food additive or supplement.

[verwijderd] 8 februari 2017 11:46

maar in de conclusie vh rapport staat toch wel iets opmerkelijks,

....LF supplementation has already many applications in food and non-food products around the world and its market continues to grow. The synthesis of rhLF via transgenic cows appears as a suitable production method that provides a large amount of rhLF with high homology with hLF. Numerous studies will be necessary to highlight the efficacy and safety of rhLF as a food additive or supplement.

komt Pharming zodoende in het vaarwater v Wessanen ??

[verwijderd] 8 februari 2017 11:46

hoi lower
best knap hoe jij die berichten altijd vind,
gr sproet.

[verwijderd] 8 februari 2017 11:55

quote:
sproetje schreef op 8 februari 2017 11:46:
hoi lower
best knap hoe jij die berichten altijd vind,
gr sproet.

Frank@ is de koene speurder in deze

[verwijderd] 8 februari 2017 12:13

www.pharming.com/science/
Transgenic Production Technology Platform (TPTP)
Pharming can produce complex therapeutic proteins in the mammary glands of rabbits or cattle and purify the protein from milk for its therapeutic application.

oud draadje:
Draadje Lactoferrine Gert50 25 nov 2005
www.iex.nl/Forum/Topic/1073135/Pharmi...

Oud onderzoek
Large scale production of recombinant human lactoferrin in the milk of transgenic cows.
2002 May
www.ncbi.nlm.nih.gov/pubmed/11981562

Dus lange adem traject blijkbaar.

Pharming heeftIn the field of transgenic technology, Pharming’s portfolio includes the following IP:

Generation and use of transgenic cattle
Milk specific expression in transgenic animals
Animals carrying large transgenes (> 50kb)
Purification of biopharmaceuticals from milk
Structure and design of transgenes for high level production
Fusion proteins for high level expression
Generation of animals using nuclear transfer technology
Oocyte activation for nuclear transfer
Transgenic antibody production
Sperm mediated gene transfer

[verwijderd] 8 februari 2017 12:32

fibrinogen on the move. jurpsy 26 aug 2006
www.iex.nl/Forum/Topic/1101291/Pharmi...
However, fibrinogen is masterful at clotting wounds while acting topically. With help from the American Red Cross and Pharming NV, a decade ago Velander began making transgenic cowsthat produce genetically engineered fibrinogen in their milk.

He incorporates the fibrinogen into a heavy-duty bandage that stops bleeding on contact. “When you put that much clotting power in the palm of your hand, it’s so powerful that you can hear the wound gushing,” Velander said.
One can make a fibrinogen bandage from the protein in human plasma, but it’s difficult toobtain large amounts of the protein. On the other hand, milk from transgenic cows contains anabundant supply. That means an animal-based fibrinogen bandage can be mass-produced at arelatively low cost.

Pharming Acquires Licenses To Key Fibrinogen Patents
Leiden, The Netherlands, June 25, 2008.
www.pharming.com/pharming-acquires-li...
Pharming has obtained exclusive licenses on recombinant fibrinogen for all indications in various territories, including North America, Europe and Japan. The licenses are to recently issued patents owned by the American Red Cross, Virginia Tech Intellectual Properties Inc and the University of North Carolina on the production of recombinant fibrinogen in milk of transgenic animals which are licensed to GTC from ProGenetics, LLC. The license agreement includes an upfront payment to GTC of approximately â‚¬ 350,000 (US$ 550,000) and a royalty on commercial sales of rhFIB. Pharming already owns several additional patents and licenses for recombinant fibrinogen and recombinant tissue sealant compositions. The acquisition of the license from GTC further broadens and strengthens Pharmingâ€™s position in this field.

[verwijderd] 8 februari 2017 18:55

quote:
lower schreef op 8 februari 2017 12:32:
fibrinogen on the move. jurpsy 26 aug 2006
www.iex.nl/Forum/Topic/1101291/Pharmi...

Leuk om dit draadje weer eens terug te lezen en leerzaam voor degenen die anderen er keer op keer op blijven wijzen dat je al je vragen bij Pharming kunt neerleggen, netjes antwoord krijgt of AVA's moet bezoeken voor goede info.
De volstrekt onbevredigende, weinig open wijze van beantwoording van het vraagstuk dat ik Pharming over de patenten en de voortgang van Fibrinogeen voorlegde, was in 2006 de druppel die voor mij de emmer vol onduidelijkheden deed overlopen. Ik kreeg het gevoel dat er zaken verhuld werden en besloot al mijn stukken te verkopen en van een LT-er voortaan alleen maar met Pharming te gaan traden. Dat was een hele goede beslissing.

ps en ga me niet vertellen dat het nu allemaal anders is ;)

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